Spinning Disk Confocal Microscope

Description:

The Spinning Disk Confocal Microscope is an Axio Observer fully motorized inverted microscope for transmitted light and epifluorescence. It is equipped with a temperature and CO2 stage incubator, making it ideal for live imaging experiments.

Applications:

Axio Observer microscopes can be used for bright field, phase contrast, differential interference contrast, VAREL contrast and PlasDIC contrast transmitted light techniques and epifluorescence.

The typical fields of application include: examination of human blood and tissue samples, observation of intracellular processes in living cell cultures, cell/cell interactions, motility, growth, measurement of potentials, drug detection, microinjection, IVF (in-vitro fertilization), toxicity studies, patch-clamp techniques, ion measurements, digital recordings, time lapse studies with automated processes, z-sectioning, deconvolution, visualization of molecular structures, Fura (Ca measurement), GFP, optical tweezers and scissors, single molecule detection, total internal reflection fluorescence (TIRF).

Specifications:

Brand: Carl Zeiss

Model: Axio Observer Z1

Objectives:

EC-plan NeoFluar 10x – bright field and epifluorescence.

EC plan Neofluar 40PxPh2 – bright field, phase contrast and epifluorescence.

Plan Apo 63x Ph3- 1.4 –DIC HCII – bright field, phase contrast, differential interference contrast, PlasDIC contrast transmitted light techniques and epifluorescence.

Plan Apo alpha 100x 1.46 – bright field and epifluorescence.

Fluorescence filters:

Set 49 ( Dapi)

Set 38 HE (GFP)

Set 31 (mCherry)

Set 50 (Cy5)

Incubation:

XL-Unit

TempModule S1

CO2 Module S1

CO2 cover HP heating P insert

Heating Insert P Lab-Tek TM compact S1

Software:

MChannel + Z+ Time

Fast Acquisition

Inside 4D

Autofocus

Uniblitz

Interactive measurements

Mark&Find

3D Deconvolution

Spinning Disk:

CSU – X1M 5000

Dicroico QDM 405-488-568-647 – Emission 482/35+Emission 562/40

Laser 488/20mW + Laser 561/20 mW