Gut bacteria such as Faecalibacterium prausnitzii and Akkermansia muciniphila have emerged from intestinal microbiome studies as candidates to the next generation of probiotics with great potential to prevent inflammatory and diet-related physiological disorders. However, these microorganism are difficult to handle because of their extreme sensitivity to traces of oxygen and so their successful use in food dietary supplements or drugs will depend on their stability and efficacy in humans. This project proposes to use microencapsulation to improve the viability and stability at atmospheric air and to achieve the intestinal delivery of these probiotics. Microfluidic-based techniques will be developed to encapsulate the model bacteria Bifidobacterium longum in a polysaccharide matrix supplemented with protective agents (antioxidants and prebiotics). Different types of microfluidic devices and formulations will be studied. The microcapsules developed will be characterized and tested for stability, viability and control delivery of the probiotic microorganism.