Leukocytes and platelet-rich fibrin (L-PRF) utilizes patient's own blood to achieve healing and tissue regeneration. It offers a broad range of cells and bioactive molecules, comprising platelets, fibrin, leukocytes and cytokines. Many studies showed possible immunological and antibacterial properties of L-PRF, but the underlying mechanisms remain unclear. The main aim of this project is to quantify inflammatory cytokines within the L-PRF exudate and evaluate its antimicrobial activity, important components in the healing process. Inflammatory cytokines profile will be analyzed by flow cytometry. L-PRF membrane and exudate will be used to evaluate antimicrobial activity using strains of bacteria isolated from own donors. Preliminary results already validated the experimental design and showed a potential effect of L-PRF exudate in activating platelets and inhibiting microbial growth of aerobic and facultative anaerobic microorganisms.