TRANSLATIONAL TOXICOLOGY LAB

ABOUT MAIN ACHIEVEMENTES TOP PAPERS 2018-2023 RESEARCH TEAM CURRENT PROJECTS PUBLICATIONS SERVICES

 Director 

ABOUT

Under construction.

 

  tt-ucibio-1htoxrun@iucs.cespu.pt  

 

MAIN ACHIEVEMENTS

One image is worth more than a thousand words in translational toxicology. The observation of thousands of poisonings allowed us to understand that the pre-analytical information provided excellent help in suspecting xenobiotics presumably related to poisonings and, as such, allowed for guidelines preparation for the selection of biological samples (e.g., 10.1080/20961790.2016.1271098), also facilitated decision-making on the most appropriate toxicological analysis techniques to be carried out, and provided a better understanding the metabolism and metabolomics as a tool to interpret results and producing better clinical reports (e.g., 10.1016/j.jflm.2018.12.005; 10.1155/2018/6852857; 10.3390/jcm10204637; 10.1080/03602532.2021.1917599; 10.3390/ph13110334). An Atlas was produced in 2021 (e.g., 10.1080/20961790.2022.2059837; 10.3390/jcm12072591; 10.3390/ijerph19148430) with more than 3000 cases of poisonings that now enables a faster diagnosis of poisonings in the world today.

The genesis of toxicology and legal medicine. In the last 17 years, it was reconstructed and studied as one of the most significant cases of Portuguese science in the medical and toxicological fields (e.g., 10.1080/20961790.2021.1898079; 10.1080/20961790.2019.1682218; 10.1080/20961790.2018.1534538). The “Rua das Flores Crime” is one of the most famous cases of poisoning that shook all European public opinion at the end of the 19th century. It demonstrated the weaknesses of the medico-legal system, attested to the importance of toxicological analyses, and prompted mind-changing and the foundations for toxicology and chemistry in Portugal and the world.

Mismanagement of plastics during the COVID-19 pandemic. The accumulation of personal protection equipment (PPE, e.g., gloves, face masks) following incorrect disposal has created challenges, not only due to its potential dissemination of the SARS-CoV-2 virus but also due to environmental impacts, including entrapment and fragmentation into microplastics (10.1016/j.cej.2020.126683; 10.1016/j.scitotenv.2021.148505). With an estimated global use of 129 billion face masks per month during the pandemic (10.1021/acs.est.0c02178), there is a need to find alternative solutions to protect populations during future outbreaks (10.3390/environments8040031). This topic has gathered widespread media coverage during the pandemic (https://www.jn.pt/..., https://sicnoticias.pt/...). 

A new method to quantify microplastics. This new method uses fluorescence to detect microplastics stained with Nile Red, counting them with the use of an original automatic tool (MP-VAT; 10.1016/j.scitotenv.2019.07.060). It has contributed to the popularity of Nile Red as a low-cost  and high throughput alternative to improve detection accuracy and has been featured in a science podcast (https://www.90segundosdeciencia.pt/...).

Environmental monitoring and ecotoxicity. Development of analytical methods for environmental monitoring programs for screening and determination of emerging contaminants namely priority substances included in the European water frame work directive (e.g., 10.1016/j.jpba.2019.03.032; 10.1016/j.scitotenv.2018.08.309) and ecotoxicity assays (e.g. 10.1002/etc.5646) for an integrative understanding of the environmental impact of contaminants. These data have been providing support for regulatory agencies in environmental management and safety (e.g. 10.1016/j.cej.2023.141617), recommendations and in promoting the sustainability and environmental health and social media outreach (10.1016/j.cej.2023.141617; https://www.publico.pt/...; https://www.rtp.pt/...).

Detection of priority bacteria resistant to last-line antibiotics across sources & countries: a One Health approach. Antimicrobial Resistance is a global Public Health threat to humans needing a holistic One Health approach. Our research uncovered Enterococcus strains harboring resistance to a last-line antibiotic (critical for humans per WHO) in commercially available European dog food. Identical strains and resistance elements were detected in food-producing animals and even hospitalized patients across Europe and China, highlighting a potential pathway for transmission between animals and humans. This work garnered significant media attention through an international press release (https://www.theguardian.com...).

 

TOP PAPERS 2018-2023

Dinis-Oliveira, R. J. (2022). One image is worth more than a thousand words: producing an atlas of medical signs for teaching clinical and forensic toxicology. Forensic Sci Res, 7(4), 815-822. doi:10.1080/20961790.2022.2059837

Prata, J. C., Paço, A., Reis, V., da Costa, J. P., Fernandes, A. J. S., da Costa, F. M., Duarte, A. C., Rocha-Santos, T. (2020). Identification of microplastics in white wines capped with polyethylene stoppers using micro-Raman spectroscopy. Food Chem, 331:127323. doi: 10.1016/j.foodchem.2020.127323.

Oravcová, V., Peixe, L., Coque, T. M., Novais, C., Francia, M. V., Literák, I., Freitas, A. R. (2018) Wild corvid birds colonized with vancomycin-resistant Enterococcus faecium of human origin harbor epidemic vanA plasmids. Environ Int, 118:125-133. doi: 10.1016/j.envint.2018.05.039.

 

RESEARCH TEAM

PhD Members 

Collaborators

PhD Students

MSc Students

Research Fellowship

Volunteers

Under construction.

Research Technician

 

CURRENT PROJECTS

FCT (Portuguese Foundation for Science and Technology) FUNDED PROJECTS
 
 
CESPU FUNDED PROJECTS
 


 

PUBLICATIONS

Rodrigues, I. C., Cristal, A. P., Ribeiro-Almeida, M., Silveira, L., Prata, J. C., Simões, R., Vaz-Pires, P., Pista, Â., & Martins da Costa, P. (2024). Gulls in Porto Coastline as Reservoirs for Salmonella spp.: Findings from 2008 and 2023. Microorganisms, 12(1), 59. https://doi.org/10.3390/microorganisms12010059

Roer, L., Kaya, H., Tedim, A. P., Novais, C., Coque, T. M., Aarestrup, F. M., Peixe, L., Hasman, H., Hammerum, A. M., & Freitas, A. R. (2024). VirulenceFinder for Enterococcus faecium and Enterococcus lactis : an enhanced database for detection of putative virulence markers by using whole-genome sequencing data. Microbiology Spectrum. https://doi.org/10.1128/spectrum.03724-23

Nuccio, A., Nogueira-Ferreira, R., Moreira-Pais, A., Attanzio, A., Duarte, J. A., Luparello, C., & Ferreira, R. (2024). The contribution of mitochondria to age-related skeletal muscle wasting: A sex-specific perspective. Life Sciences, 336, 122324. https://doi.org/10.1016/j.lfs.2023.122324

Pérez-Pereira, A., Carrola, J. S., Tiritan, M. E., & Ribeiro, C. (2024). Enantioselectivity in ecotoxicity of pharmaceuticals, illicit drugs, and industrial persistent pollutants in aquatic and terrestrial environments: A review. Science of The Total Environment, 912, 169573. https://doi.org/10.1016/j.scitotenv.2023.169573

Almeida-Nunes, D. L., Silvestre, R., Dinis-Oliveira, R. J., & Ricardo, S. (2024). Enhancing Immunotherapy in Ovarian Cancer: The Emerging Role of Metformin and Statins. International Journal of Molecular Sciences, 25(1), 323. https://doi.org/10.3390/ijms25010323

Reis-Mendes, A., Ferreira, M., Padrão, A. I., Duarte, J. A., Duarte-Araújo, M., Remião, F., Carvalho, F., Sousa, E., Bastos, M. L., & Costa, V. M. (2024). The Role of Nrf2 and Inflammation on the Dissimilar Cardiotoxicity of Doxorubicin in Two-Time Points: a Cardio-Oncology In Vivo Study Through Time. Inflammation, 47(1), 264–284. https://doi.org/10.1007/s10753-023-01908-0

Carvalho, C., Barbosa, D. J., Celestino, R., Zanin, E., Xavier Carvalho, A., & Gassmann, R. (2024). Dynein directs prophase centrosome migration to control the stem cell division axis in the developing C. elegans epidermis. GENETICS. https://doi.org/10.1093/genetics/iyae005

Valente-Santos, J., Vitorino, R., Sousa-Mendes, C., Oliveira, P., Colaço, B., Faustino-Rocha, A. I., Neuparth, M. J., Leite-Moreira, A., Duarte, J. A., Ferreira, R., & Amado, F. (2024). Long-Term Exposure to Supraphysiological Levels of Testosterone Impacts Rat Submandibular Gland Proteome. International Journal of Molecular Sciences, 25(1), 550. https://doi.org/10.3390/ijms25010550

Moreira-Pais, A., Ferreira, R., Oliveira, P. A., Neuparth, M. J., Appell, H.-J., & Duarte, J. A. (2024). Glycolysis and skeletal muscle plasticity: lactate as a key signaling molecule. In Glycolysis (pp. 51–74). Elsevier. https://doi.org/10.1016/B978-0-323-91704-9.00013-6

 

AFFILIATION

English affiliation:

- Associate Laboratory i4HB - Institute for Health and Bioeconomy, University Institute of Health Sciences - CESPU, 4585-116 Gandra, Portugal.

- UCIBIO - Applied Molecular Biosciences Unit, Translational Toxicology Research Laboratory, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal.

 Portuguese affiliation:

- Laboratório Associado i4HB - Instituto de Saúde e Bioeconomia, Instituto Universitário de Ciências da Saúde - CESPU, 4585-116 Gandra, Portugal.

- UCIBIO - Unidade de Biociências Moleculares Aplicadas, Laboratório de Investigação em Toxicologia Translacional, Instituto Universitário de Ciências da Saúde (1H-TOXRUN, IUCS-CESPU), 4585-116 Gandra, Portugal.

 

SERVICES

Under construction.